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1.
Pesticidas ; 20(1): 1-6, jan.-dez. 2010. ilus, tab, graf
Article in English | LILACS | ID: lil-587601

ABSTRACT

Mutants of Trichoderma harzianum Rifai, obtained after ultraviolet (UV) light exposure, showed high resistant to the fungicide benomyl. A mutant (2B6)was capable of degrading carbendazim, other fungicide of the benzimidazole fungicide. This mutant degraded 41.5% of the molecule within five days. This and others mutants (2B1 and 2B2) presented variation in size and frequency of uni-nucleated and/ or bi-nucleated spores compared to the wild type. Four primers generated RAPDs patterns that allowed the mutant to be differentiated from the wild-type. It is concluded that using UV mutagenization, it is feasible to obtain strains of T. harzianum with improved pesticide degradation ability.


Subject(s)
Biological Availability , Biotransformation , Fungi , Pesticides
2.
Braz. j. microbiol ; 37(4): 417-419, Oct.-Dec. 2006. ilus
Article in English | LILACS | ID: lil-442186

ABSTRACT

An Aspergillus terreus strain showed in vitro antagonistic activity against the plant pathogen Sclerotinia sclerotiorum (Lib.) de Bary. The interaction between A. terreus and sclerotia revealed that the mycoparasite sporulated abundantly on the sclerotial surface. Cell breakdown due to host cell wall disruption was observed in inner rind cells, by a scanning electron microscopy.


Uma linhagem de Aspergillus terreus mostrou forte atividade parasítica contra Sclerotinia sclerotiorum. Interações entre o patógeno e o antagonista revelaram que A. terreus esporulou profusamente sobre os escleródios. Quando visto em microscopia eletrônica de varredura, o antagonista mostra-se rompendo e lisando a parede celular e penetrando o interior do escleródio, onde se estabelece no tecido medular.


Subject(s)
Aspergillus , Ascomycota/physiology , In Vitro Techniques , Pest Control, Biological , Soil Microbiology , Ascomycota/isolation & purification , Aspergillus/isolation & purification , Cell Wall , Microscopy, Electron, Scanning
3.
Braz. j. microbiol ; 35(4): 330-332, Oct.-Dec. 2004. ilus
Article in English | LILACS | ID: lil-402618

ABSTRACT

Estudos morfológicos de conídios de Trichoderma harzianun, um agente de biocontrole da vassoura-de-bruxa do cacaueiro, foram feitos sob microscopia eletrônica de varredura com emissão de campo. Características da superfície de conídios do fungo mostraram ser rugosas quando observadas em alta magnificação; fato esse impossível de ser visualizado por microscopia ótica. Também foram observados, com freqüência, massas de conídios completamente envolvidos por material mucilaginoso e detalhes de células ramificadas dicotomicamente que formam as pústulas.


Subject(s)
Cacao , Conidiobolus , In Vitro Techniques , Mitosporic Fungi , Pest Control, Biological , Trichoderma , Microscopy, Electron, Scanning
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